B.S., Biochemistry, The State University of New York - University at Buffalo
M.S., Chemistry, University of Rochester
PhD, Biochemistry/Biophysics, Johns Hopkins
Post-doc, Pharmacology, University of North Carolina - Chapel Hill
Dr. Robert K. DeLong serves as associate professor in the Nanotechnology Innovation Center Kansas State within the Department of Anatomy and Physiology. The overall thrust in his research program is the impact of nanomaterials on the structure, function and delivery of nucleic acids and proteins. Research has received support from National Institute of Health, National Science Foundation, Howard Hughes Medical Institute and the American Physiology Society. His group has a keen interest in the anti-cancer properties of nanoparticles when combined with nucleic acid therapeutics (NAT) and anticancer protein (ACP) and also studies the biochemical and immunological activity of nanoparticles and their application as new fluorescence or bioluminescence-enhanced imaging agents. Dr. DeLong is a co-founder of the Journal of Biomedical Nanotechnology and continues to serve on its executive editorial board and is currently serving as a guest editor of the journal, Nanomaterials, in a special issue focusing on their anticancer, biochemical and immunological activity. He has 14 patents or patent applications, one textbook published by Academic Press/Elsevier on biomolecular interactions for the molecular biology and biochemistry laboratory, and has published numerous book chapters and review articles on RNA nanoparticles, their characterization and delivery. Some highlights of the publications and patents generated by the group since he returned to academia from industry are listed below:
Representative Research Publications
DeLong RK, Mitchell JA, Morris RT, Comer J, Hurst MN, Ghosh K, Wanekaya A, Mudge M, Schaeffer A, Washington LL, Risor-Marhanka A, Thomas S, Marroquin S, Lekey A, Smith JJ, Garrad R, Aryal S, Abdelhakiem M, Glaspell GP. Enzyme and cancer cell selectivity of nanoparticles: inhibition of 3-d metastatic phenotype and experimental melanoma by zinc oxide” J Biomed Nanotechnol 13, 221-231, 2017.
Ramani M, Mudge MC, Morris RT, Zhang Y, Warcholek SA, Hurst MN, Riviere JE, DeLong RK. Zinc oxide nanoparticle-poly I:C RNA complexes: implication as therapeutics against experimental melanoma. Mol Pharm 2017.
DeLong RK, Curtis CB. Toward RNA nanoparticle vaccines: synergizing RNA and inorganic nanoparticles to achieve immunopotentiation.Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2016 Jun 16. doi: 10.1002/wnan.1415. [Epub ahead of print]
Delong RK, Hurst MN, Aryal S, Inchun NK. Unique Boron Carbide Nanoparticle Nanobio Interface: Effects on Protein-RNA Interactions and 3-D Spheroid Metastatic Phenotype. Anticancer Research 36(5): 2097-103, 2016.
Manani GN, Spidle RT, Bhaumik A, Ghosh K, Pandey RR, Chusuei CC, Delong R, Wanekaya AK. Novel aqueous fabrication and characterization of gold coated cobalt nanoparticles. Current Bionanotechnology 1(2): 95 – 101, 2016.
Dean JM, DeLong RK. A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells. Methods Mol Biol. 2015;1297:187-96. doi: 10.1007/978-1-4939-2562-9_13. PMID:25896004
Wesley Dowler, Jacob Robison, Jonathan Fury, Xiaozheng Dou, Robert DeLong & Reza Sedaghat-Herati. Synthesis, Characterization and Applications of Poly(ethylene glycol)-block-poly(ether-sulfide) Dendrimers. Journal of Macromolecular Science, Part A: Pure and Applied Chemistry Volume 52, Issue 1, 2015.
Bhaumik A, Shearin AM, Delong R, Wanekaya A, Ghosh K. Probing the Interaction at the Nano-Bio Interface Using Raman Spectroscopy: ZnO Nanoparticles and Adenosine Triphosphate Biomolecules.J Phys Chem C Nanomater Interfaces. 2014 Aug 14;118(32):18631-18639. Epub 2014 Jul 29. PMID:25152799
Venuturumilli, Sindhuri; Dean, John M.; Hurst, Miranda; DeLong, Robert K. Poly-Acrylic and Nucleic Acid Cobalt Hexamine Nanocomplexes: Characterization, RNase Protection and Inhibition of In Vitro Tumorigenicity in Soft Agar. Nanoscience and Nanotechnology Letters, Volume 6, Number 11, November 2014, pp. 976-981(6).
Vishala S Ramyah Aaryasomayajula, Tiffany Severs, Kartik Ghosh, Robert DeLong, Xianfeng Zhang, Saikat Talapatraand Adam K Wanekaya. Assembly of a Dual Aptamer Gold Nanoparticle Conjugate Ensemble in the Specific Detection of Thrombin when Coupled with Dynamic Light Scattering Spectroscopy. J Nanomed Nanotechnol 5:210 (2014). doi: 10.4172/2157-7439.1000210.
Reyes-Reveles J, Sedaghat-Herati R, Gilley DR, Schaeffer AM, Ghosh KC, Greene TD, Gann HE, Dowler WA, Kramer S, Dean JM, Delong RK. mPEG-PAMAM-G4 nucleic acid nanocomplexes: enhanced stability, RNase protection, and activity of splice switching oligomer and poly I:C RNA. Biomacromolecules. 2013 Nov 11;14(11):4108-15. doi: 10.1021/bm4012425. Epub 2013 Oct 28. PMID:24164501
Wayu MB, Spidle RT, Devkota T, Deb AK, Delong RK, Ghosh KC, Wanekaya AK, Chusuei CC. Morphology of hydrothermally synthesized ZnO nanoparticles tethered to carbon nanotubes affects electrocatalytic activity for H2O2 detection.Electrochim Acta. 2013 May 1;97:99-104. PMID:25684785
Delong RK, Risor A, Kanomata M, Laymon A, Jones B, Zimmerman SD, Williams J, Witkowski C, Warner M, Ruff M, Garrad R, Fallon JK, Hickey AJ, Sedaghat-Herati R. Characterization of biomolecular nanoconjugates by high-throughput delivery and spectroscopic difference.Nanomedicine (Lond). 2012 Dec;7(12):1851-62. doi: 10.2217/nnm.12.70. Epub 2012 Sep 3. PMID:22943129
Parker-Esquivel B, Flores KJ, Louiselle D, Craig M, Dong L, Garrad R, Ghosh K, Wanekaya A, Glaspell G, DeLong RK. Association of poly I:C RNA and plasmid DNA onto MnO nanorods mediated by PAMAM.Langmuir. 2012 Feb 28;28(8):3860-70. doi: 10.1021/la203998r. Epub 2012 Feb 14. PMID:22220841
Barber S, Abdelhakiem M, Ghosh K, Mitchell L, Spidle R, Jacobs B, Washington L, Li J, Wanekaya A, Glaspell G, DeLong RK. Effects of nanomaterials on luciferase with significant protection and increased enzyme activity observed for zinc oxide nanomaterials.J Nanosci Nanotechnol. 2011 Dec;11(12):10309-19. PMID:22408903