The Olympus IX73 inverted fluorescent microscope system allows for live cell imaging in a temperature- and CO2-controlled environment. Labor-intense localization is aided by the ProScan™III motorized stage, three-dimensional stage joystick, and image tiling and z-stacking. The microscope can be autofocused using the CellSens Dimension software. It has DAPI, FITC, TXRED, CY3, CY5, and QD655 filter sets for fluorescent imaging. The images acquired are high resolution bright field images, enabling sensitive fluorescence detection, due to the DP80 12.7MP dual CCD camera, which combines both a color and a monochrome sensor. This microscope can be operated in differential interference contrast (DIC mode) and has a three position coded magnification changer (1X, 1.6X, 2X).
Projects are initiated by a meeting of the user, principal investigator, Core Director and Core Manager. Contact Mr. Tej Shrestha.
The Core provides individual training.
Trained users coordinate instrument use via the Scheduler.
Nanotechnology core facility equipment use is currently free for properly trained persons, with approval of the lab manager. However, a fee structure will be implemented long-term. This fee structure is expected to be similar to that of the other core labs on campus.